Anti-Human Antibody- 30 nm Gold Nanoparticles Conjugate (O.D 20)
Product: Anti-Human Antibody- 30 nm Gold Nanoparticles Conjugate
Quality Control: Each lot of NANOSHEL Anti-Human Antibody- 30 nm Gold NanoparticlesConjugate (O.D 20) was tested successfully.
|Gold Nanoparticle Core Size||30nm|
|Optical Density||3OD (conc. 1.79E+13 particles/ml)|
|Conjugated Antibody||Affinity Purified Anti-Human IgG (H+L)|
|Supplied in||20mM Tris (pH 8.0), 150mM NaCl, 20% glycerol (v/v), 1% BSA|
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Dr. Stephen Parezo PhD (Polytechnic University of Milan, Italy)
Antibody Conjugation Kits provide a method to conjugate your antibodies quantum dot nanocrystals. The kit contains amine-derivatized, PEG-coated nanocrystals and the amine-thiol crosslinker, SMCC. In addition to antibodies, other thiol-containing molecules can be coupled to these nanocrystals using this kit. Primary antibodies labeled using these methods are excellent reagents in flow cytometry, fluorescence imaging, western blots, and more. The kits are available in eight nanocrystal colors and supply the reagents needed for two labeling and separation reactions.
Dr. Ms Celia Brewerton ( Technological University of N. of Aguascalientes, Mexico)
The nanocrystal is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of detection reagents. Nanocrystals are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The 705 and 800 nanocrystals, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.
Dr. Rolf Larsson PhD (Karlstad University, Karlstad, NärkeNärke, Sweden)
Extremely photostable and bright fluorescence (very high fluorescence quantum yield) Efficiently excited with single-line excitation sources. Narrow emission profiles, large Stokes shift Nanocrystals are our brightest fluorescent option for labeling, with molar extinction coefficients several orders of magnitude brighter than our brightest organic dyes. Nanocrystals are an excellent choice for primary antibody based detection or for rare event and even single-molecule studies. By avoiding secondary detection, primary antibody–based approaches compress workflows in addition to allowing matched isotype/species antibodies to be used.
Dr. Dewei Leong Phd (Bandung Institute of Technology, Bandung, Indonesia)
605 quantum dot has emission maxima of ~605 nm. Labeled proteins typically ready to use in 4–5 hours.Optimized for ~300 μg of whole IgG or polyclonal Fab fragments. Requires the antibody or protein be purified and reduced prior to labeling
Dr. Villy Petersen Professor (Sebastian Kolowa Memorial University, Lushoto, Tanzania)
Gold conjugates are suitable for use in immunoblotting, light microscopy, and electron microscopy applications. Sensitive technique (100pg or less in dot blotting when used together with our silver enhancement kit) Provides a permanent label. Store undiluted in storage buffer at 2-8oC. Stable for 4 months if stored as specified. Storage of conjugate at working dilution may result in performance loss.